熊勇华老师（研究员/博士生导师）

• 姓名：熊勇华

• 职称：研究员（博士生导师）

• 电子邮箱：yhxiongchen@163.com

• 教育经历：

1988年就读于江西大学食品化学专业，1992年获学士学位；

2002年在南昌大学攻读食品科学专业的博士研究生，2004年6月获博士学位；

• 研究工作经历：

1992年9月至1997年9月，江西中德联合研究院任助教；

1997年10月至2002年9月，江西中德联合研究院任讲师；

2002年10月至2007年9月，南昌大学中德联合研究院任副研究员；

2007年8月-2008年8月获国家留学基金委资助在美国阿肯色大学教授实验室进行为期一年的博士后研究。

2007年10月至今，南昌大学中德联合研究院任研究员；

2012年10月至今，食品科学与技术国家重点实验室固定成员；

2015年10月-2016年4月于美国麻省大学阿莫斯特分校Rotello实验室高级访问交流半年。

• 在研主持课题：

1. 国家重点基础研究发展计划项目课题973计划--储粮真菌毒素早期监测预警基础（2013CB127804）；

2. 国家自然科学基金面上项目--基于大体系下(50mL)纳米免疫磁珠高效富集单核增生李斯特菌的方法学研究；

3. “十二五”国家科技支撑计划子课题--食品中毒因子解析技术研究（2011BAK10B06）；

4. 科技部“食品科学与技术国家重点实验室”自由探索课题--建立大体系下纳米磁珠富集单核增生李斯特菌的磁分离模型；

5. 江西省教育厅落地计划“水相容性展青霉素表面分子印迹磁性纳米粒子”的制备及应用研究。

• 近三年主持验收课题：

1. 江西省青年科学家（井冈之星）培养对象（2013-06-20）；

2. 留学人员科技活动择优项目（2012-10-10）；

3. 教育厅产学研合作项目（2012-12-30）；

4. 国家863计划“具有高黏附、拮抗和抗炎症能力的双歧杆菌的高效筛选和功能基因的定位”（2011-05-09，第二主持）。

• 获得专利情况：

1. 桔霉素免疫层析试纸条及其制备方法与应用。发明专利号:ZL03135011.9;授权时间:2008,7,23.

2. 展青霉素免疫层析试纸条及其制备方法与应用。发明专利号:ZL03160299.1;授权时间:2007,2,14.

3. 抗轮状病毒鸡卵黄免役球蛋白产品。发明专利号:ZL98107254.2;授权时间:2006,12,20.

4. 抗绿脓杆菌鸡卵黄免役球蛋白的产品及其应用。发明专利号:ZL98116441.2;授权时间:2003,2,12.

5. 抗幽门螺杆菌鸡卵黄免役球蛋白的产品及其应用。发明专利号:ZL99117588.3;授权时间:2001,1,9.

6. 一种热启动耐高温DNA聚合酶及其制备方法。发明专利号:200710065121.4;授权时间:2010,6,30.

7. 检测残留兽药的荧光微球免疫层析试纸条及其制备方法。发明专利号:200710166166.0;授权时间:2012,8,8.

8. 一种荧光微球免疫层析试纸条的制备方法及其定量方法。发明专利号:200910117820.8;授权时间:2012,06,27.

• 近三年发表SCI论

1. Xiaolin Huang, Shengnan Zhan, Hengyi Xu, Xianwei Meng, Yonghua Xiong* and Xiaoyuan Chen*. Ultrasensitive fluorescence immunoassay for detection of ochratoxin A using catalase-mediated fluorescence quenching of CdTe QDs. Nanoscale, 2016, 8, 9390–9397. IF=7.76

2. Xiaolin Huang, Zoraida P. Aguilarc, Hengyi Xu*, Weihua Lai, Yonghua Xiong*. Membranebased lateral flow immunochromatographic strip with nanoparticles as reporters for detection: A review. Biosensors and Bioelectronics. 2016,75:166–180. IF=7.476

3. Xiaolin Huang, Rui Chen, Hengyi Xu,* Weihua Lai, and Yonghua Xiong*. Nanospherical brush as catalase container for enhancing the detection sensitivity of competitive plasmonic ELISA. Anal. Chem. 2016, 88, 1951−1958. IF=5.83.

4. Yi Liang, Xiaolin Huang, Ruijin Yu, Yaofeng Zhou, Yonghua Xiong. Fluorescence ELISA for sensitive detection of ochratoxin A based on glucose oxidase-mediated fluorescence quenching of CdTe QDs. Analytica Chimica Acta, 2016, 936:195-201. IF = 4.712

5. Juan Li, Hong Duan, Peng Xu, Xiaolin Huang and Yonghua Xiong*. Effect of different-sized spherical gold nanoparticles grown layer by layer on the sensitivity of an immunochromatographic assay. RSC Adv.. 2016, 6:26178–26185. IF=3.289

6. Shengnan Zhan, Xiaolin Huang, Rui Chen, Juan Li, Yonghua Xiong*. Novel fluorescent ELISA for the sensitive detection of zearalenone based on H2O2-sensitive quantum dots for signal transduction. Talanta, 2016, 158:51–56. IF=4.035

7. Xuqiang Zou, Chaochao Chen, Xiaolin Huang, Xuelan Chen, Lv Wang, Yonghua Xiong*. Phage-free peptide ELISA for ochratoxin A detection based on biotinylated mimotope as a competing antigen. Talanta, 2016,146(1): 394–400. IF=4.035

8. Yan Mao, Xiaolin Huang, Sicheng Xiong, Hengyi Xu*, Zoraida P. Aguilar, Yonghua Xiong*. Large-volume immunomagnetic separation combined with multiplex PCR assay for simultaneous detection of Listeria monocytogenes and Listeria ivanovii in lettuce. Food Control. 2016, 59:601-608. IF=3.388

9. Peng Xu, Juan Li, Xiaolin Huang, Hong Duan, Yanwei Ji and Yonghua Xiong*. Effect of the tip length of multi-branched AuNFs on the detection performance of immunochromatographic assays. Anal. Methods, 2016, 8, 3316–3324. IF=1.915

10. Liang Guo, Yuyan Liang, Xuelan Chen, Wei Xu1, Kesheng Wu, Hua Wei, and Yonghua Xiong*. Effective Removal of Tetracycline from Aqueous Solution by Organic Acid-coated Magnetic Nanoparticles. Journal of Nanoscience and Nanotechnology. 2016, 16(3):2218-2226. IF=1.338

11. Rui Chen, Xiaolin Huang, Hengyi Xu, Yonghua Xiong,* and Yanbin Li. Plasmonic enzyme-linked immunosorbent assay using nanospherical brushes as a catalase container for colorimetric detection of ultralow concentrations of listeria monocytogenes. ACS Appl. Mater. Interfaces 2015, 7, 28632−28639. IF=7.145

12. Xiaolin Huang; Zhaodi Xu; Yan Mao; Yanwei Ji; Hengyi Xu*; Yonghua Xiong*; Yanbin Li. Gold nanoparticle-based dynamic light scattering immunoassay for ultrasensitive detection of Listeria monocytogenes in lettuces. Biosensors and Bioelectronics, 2015, 66, 184-190. IF=7.476

13. Minliang Chen, Xuelan Chen*, Fang Wan, Bin Zhang, Jincong Chen and Yonghua Xiong*. Effect of Tween 40 and DtsR1 on L-arginine overproduction in Corynebacterium crenatum. Microb Cell Fact 2015,14:119.IF=4.22

14. Ying Xiong, Zhui Tu, Xiaolin Huang, Bing Xie, Yonghua Xiong* and Yang Xu.  Magnetic beads carrying poly(acrylic acid) brushes as “nanobody containers” for immunoaffinity purification of aflatoxin B1 from corn samples. RSC Adv., 2015,5, 77380-77387. IF=3.84

15. Yanwei Ji, Meiling Ren, Yanping Li, Zhibing Huang, Mei Shu, Hongwei Yang,Yonghua Xiong*, Yang Xu*. Detection of aflatoxin B1 with immunochromatographic test strips: Enhanced signal sensitivity using gold nanoflowers. Talanta, 2015,142:206–212.IF=3.51

16. Jun Shen, Yaofeng Zhou, Fen Fu, Hengyi Xu*, Jiaofeng Lv, Yonghua Xiong*, Andrew Wang. Immunochromatographic assay for quantitative and sensitive detection of hepatitis B virus surface antigen using highly luminescent quantum dot-beads. Talanta, 2015,142:206–212. IF=3.51

17. Hong Duan; Xuelan Chen; Wei Xu; Jinhua Fu; Yonghua Xiong*; Andrew Wang. Quantum-Dot submicrobead-based immunochromatographic assay for quantitative and sensitive detection of zearalenone. Talanta, 2015,132:126-13. IF=3.51

18. Bin Zhang, Fang Wan, Yu-Lou Qiu, Xue-Lan Chen*, Li Tang, Jin-Cong Chen, Yong-Hua Xiong*. Increased L-arginine Production by Site-directed Mutagenesis of N-acetyl-L-glutamate Kinase and proB Gene Deletion in Corynebacterium crenatum. 2015, 28(12):864-874. IF=1.65

19. 周耀锋, 熊斯诚, 江湖, 段宏, 熊勇华*, Andrew Wang. 量子点荧光微球免疫层析试纸条定量检测玉米中赭曲霉毒素A. 分析化学. 2015, 43: 1837~1843. IF=0.7

20. Hong Duan; Xuelan Chen; Hu Jiang; Jun Shen; Shnegming Dong; Yonghua Xiong*; Andrew Wang, Quantum-dot Submicrobead-Based Immunochromatographic Strip for Rapid and Quantitative Detection of Plasmodium Falciparum. Chinese Journal of Analytical Chemistry, 2015,3:338-343. IF= 0.7

21. Wei, Xu; Yonghua Xiong*; Weihua Lai; Yang Xu*; Changming Li; Mingyong Xie, A homogeneous immunosensor for AFB1 detection based on FRET between different-sized quantum dots. Biosensors and Bioelectronics, 2014, 56, 144-150. IF=6.45

22. Meiling Ren; Hengyi Xu; Xiaolin Huang; Min Kuang; Yonghua Xiong*; Hong Xu; Yang Xu; Hongyu Chen; Andrew Wang*, Immunochromatographic Assay for Ultrasensitive Detection of Aflatoxin B1 in Maize by Highly Luminescent Quantum Dot Beads. ACS Applied Materials & Interfaces, 2014, 6(16):14215-14222. IF=6.73

23. Kesheng Wu; Liang Guo; Wei Xu; Hengyi Xu; Zoraida P. Aguilar; Guomao Xu; Weihua Lai; Yonghua Xiong*; Yiqun Wan, Sulfonated polystyrene magnetic nanobeads coupled with immunochromatographic strip for clenbuterol determination in pork muscle. Talanta. 2014,129: 431-437. IF= 3.51

24. Shengjie Li; Tingtao Chen; Feng Xu; Suqin Dong; Hengyi Xu; Yonghua Xiong*; Wei Hua*, The beneficial effect of exopolysaccharides from Bifidobacterium bifidum WBIN03 on microbial diversity in mouse intestine. Journal of the Science of Food and Agriculture, 2014, 94(2):256-264. IF= 1.88

25. Xuelan Chen; Bin Zhang; Li Tang; Haitao Jiao; Hengyi Xu; Feng Xu; Hong Xu; Hua Wei; Yonghua Xiong*, Expression and Characterization of ArgR, An Arginine Regulatory Protein in Corynebacterium crenatum. Biomedical and Environmental Sciences, 2014, 27(6):436-443. IF= 1.65

26. Meiling Ren; Xuelan Chen; Chaohui Li; Bo Xu; Wenjuan Liu; Hengyi Xu; Yonghua Xiong*. Lateral Flow Immunoassay for Quantitative Detection of Ractopamine in Swine Urine. Biomedical and Environmental Sciences, 2014, 27(2):134-137. IF=1.65